Optimization Of Saponin Extraction Conditions From Ampelozizyphus Amazonicus By Using Experimental Design And Surface Response Methodology [otimização Das Condições De Extração De Saponinas Em Ampelozizyphus Amazonicus Usando Planejamento Experimental E Metodologia De Superfície De Resposta]

摘要

METHODOLOGY. This works describes the use of experimental design and surface response methodology for optimization of saponin extraction from Ampelozizyphus amazonicus. For this purpose, a method employing extraction based on maceration assisted by ultrasound technique was utilized. The following factors were studied: extraction length of time and solvent composition. The total saponin was determined by using a gravimetric method and the results expressed by their relative proportion to total crude extract. For the specific condition, 60% hydro-alcoholic solution and 18 minutes extraction length of time has shown the best results. This method can be useful for extraction of substances with biological importance.